Vapes Found 3,000 Times Dirtier Than Toilet Seats in Shocking Study
A new laboratory study reveals that vape mouthpieces can harbour 3,000 times more bacteria and fungi than the average public toilet seat. The warm, moist environment creates an ideal breeding ground for dangerous microorganisms.
Key Takeaways
- Vape mouthpieces showed 150,000 colony-forming units of bacteria/fungi after just 3 days
- This is 3,000 times more contaminated than typical public toilets
- Researchers identified E. coli, Staphylococcus, and other harmful microbes
- Experts recommend cleaning vapes every 3 days with antibacterial wipes
Rapid Microbial Growth Observed
Scientists at BioLabTests partnered with nicotine retailer Haypp to test a strawberry ice flavoured Lost Mary vape over two weeks. They observed explosive microbial growth between days 2-3, with colonies multiplying thousands of times.
By day three, the mouthpiece contained approximately 150,000 individual colony-forming units (CFUs) – the maximum measurable by the lab and 3,000 times more than the 50 CFUs typically found on public toilet seats.

Dangerous Microbes Identified
Laboratory analysis revealed several concerning microorganisms on the tested vape:
- E. coli – indicates possible faecal contamination
- Staphylococcus – can cause infections despite being normally harmless
- Enterococcus – intestinal bacteria that persists on surfaces
- Yeast and mould – can cause respiratory irritation
- Bacillus – commonly found in dust and air
Reynold Mpofu, microbiologist at BioLabTests, explained: “Laboratory analysis revealed that the mouthpiece is the dirtiest component of the vape. This isn’t surprising given that the human mouth harbours approximately 700 species of bacteria.”
How Contamination Occurs
Researchers believe microbes transfer from unwashed hands, surfaces, and environments to vapes. The devices are frequently handled and placed on various surfaces, allowing germs to latch on.
“The findings confirmed the presence of bacteria, including E. coli and Enterococcus, on the vape body, likely arising from inadequate hand hygiene or transfer from bathroom environments,” Mpofu said.

Cleaning Recommendations
Experts strongly advise cleaning vapes more frequently than commonly recommended. Markus Lindblad of Haypp suggests: “We recommend wiping down both the mouthpiece and body every three days with a cloth dampened in antibacterial cleaner or using an alcohol wipe. Most advice online recommends once a week; however, our research shows this is far too long between cleans.”
For vapes with interchangeable pieces, components should be removed and cleaned individually to ensure no bacteria remains.
Vaping Context and Health Concerns
In the UK, 5.4 million people now vape regularly, outnumbering the 4.9 million cigarette smokers. While e-cigarettes contain fewer toxic chemicals than traditional cigarettes, they typically contain addictive nicotine.
Research has linked vaping with heart failure, lung disease, and gum disease. The World Health Organisation notes e-cigarettes are particularly risky for teenage brain development and can damage babies in the womb.
With an estimated 40,000 vape flavours available worldwide, proper hygiene practices are increasingly important for the millions of regular users.









